Western blot was performed using Anti-Transferrin Recombinant Rabbit Monoclonal Antibody (Product # MA5-30565) and a 80 kDa band corresponding to Transferrin was observed across the samples except in HeLa and HeLa conditioned media in Fig. a and was also observed in Hep G2 cells upon treatment with secretion blockers (FLI-06 and PTI) in Fig. b. An uncharacterized band at ~70 kDa was observed across the samples. The expression of Transferrin was not observed in K-562 as reported in literature. An additional uncharacterized band was observed at ~60 kDa across all the samples tested. Whole cell extracts (30ug lysate) of Hep G2 (Lane 1), HeLa (Lane 2), 7 ul of Hep G2 conditioned media (Lane 3), 7 ul of HeLa conditioned media (Lane 4) and 2 ul of Human Plasma (Lane 5) in Fig. a; whole cell extracts (30ug lysate) of Hep G2 (Lane 1) Hep G2 treated with FLI-06 (10uM for 4hr) (Lane 2), Hep G2 treated with PTI (1X for 4hr) (Lane 3), K-562 (Lane 4), K-562 treated with FLI-06 (10uM for 4hr) (Lane 5) and K-562 treated with PTI (1X for 4hr) (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP.